Isolated Hybrid Endosperm of Mimulus guttatus

The angiosperm endosperm serves as a nutritive tissue to the developing embryo. The endosperm thus plays a pivotal role in seed development, and dysregulation of endosperm development represents a key postzygotic barrier to reproduction. This property of hybrid seed lethality through endosperm failure is well documented as a common reproductive barrier in the Mimulus genus. To perform targeted expression analysis of the Mimulus endosperm, we performed RNA-seq analysis on isolated endosperm from a cross between two Mimulus guttatus accesions. Two Mimulus guttatus accessions (CSS4 and IM767) were crossed. Parental plants were grown in 21 degree Celsius growth chambers and paired for reciprocal crosses. Hand dissected endosperm at 9-10 DAP.