Sequencing of mouse gut microbiome with Nanopore Sequencing for whole genome analysis of DNA methylation.

Fecal pellets from a male 12-week-old NOD/shiltj mouse were collected before and after antibiotic treatment with Tylosin. DNA samples were extracted and sequenced with Nanopore sequencing. For each microbiome sample, nanopore sequencing raw signal is compared between the native and the WGA datasets to bin metagenomic contigs by genome of origin. Those information are also leverage to detect, classify, and fine-mapped DNA methylation across the genomes.