Effect of hepatocyte-specific PPARgamma (PPARg)expression and diet-induced NASH in the liver transcriptome of male and female mice with pre-stablished diet-induced obesity

Adult PPARg floxed male and female mice were fed a high fat diet (HFD) for 16 weeks to induce obesity. Half of these mice were then injected with AAV8-TBG-Cre to knockout PPARg in hepatocytes. The remaining half were injected with AAV8-TBG-Null to generate control mice. After two weeks, mice fed the HFD were either maintained on this diet or switched to a high fat, high cholestrol, high fructose (HFCF) diet for an additional 16 weeks. This study was designed to examine whether the loss of hepatocyte PPARg in mice with established obesity would alter the liver transcriptomics in a PPARg dependent manner when the mice are fed a HFD or a HFCF diet. There are 10 groups of mice which are catagorized by there gender (Male [M], Female [F]), diet (Low fat diet [L], High fat diet [H], High fat, cholesterol and fructose [N], and PPARgamma expression (Control [C], Hepatocyte-specific KO [K]). For MALE mice: Male, HIgh fat diet, control mice [MHC]: 6 mice, MHC876, MHC878, MHC920, MHC922, MHC958, MHC962; Male, HIgh fat diet, PPARgamma knockout mice [MHK]: 6 mice, MHK877, MHK879, MHK923, MHK959, MHK961, MHK963. Male, High fat, cholesterol and fructose diet, control mice [MNC]: 6 HFCF mice: MNC892, MNC894, MNC908, MNC910, MNC948, MNC950; Male, High fat, cholesterol and fructose diet, PPARgamma knockout mice mice [MNK]: 6 mice, MNK893, MNK911, MNK949, MNK970, MNK972, MNK974.; For FEMALE; Female, low fat diet, control mice [FLC]: 4 mice, FLC1152, FLC1156, FLC1091, FLC1093; Female, low fat diet, PPARgamma knockout mice [FLK]: 4 mice, FLK1099, FLK1110, FLK1102, FLK1153; Female, High fat diet, Control mice [FHC]: 4 mice, FHC1137, FHC1151, FHC1136, FHC1158; Female, High fat diet, PPARgamma knockout mice [FHK]: 4 mice, FHK1134, FHK1157, FHK1135, FHK1160; Female, High fat, cholesterol, fructose, control mice [FNC]: 4 mice, FNC1117, FNC1118, FNC1119, FNC1142; Female, High fat, cholesterol, fructose, PPARgamma knockout mice [FNK]: 4 mice FNK1084, FNK1085, FNK1116, FNK1140.