MicroRNAs as a therapeutic target in IgA nephropathy in Indian population

Immunoglobulin A Nephropathy (IgAN) is the most occurring glomerular disease with rapid development to End Stage Renal Disease requiring renal replacement therapy. Genome-wide studies suggest geographical variations in genetic susceptibility to IgAN and disease progression have been shown to play a vital role in physio pathological state. In this study, we have identified new microRNAs allied with IgAN in Indian population. The miRNA was isolated from kidney biopsies of IgAN patients and healthy control (healthy tissue from patients with renal cell carcinoma). Small RNA library preparation was performed, followed by Next Generation Sequencing on the ION S5 Torrent at 1x50 base pairs. Later the miRNA expression analyses were carried and obtained high-quality reads from BAM file. The sequence of miRNA reads was mapped to reference Human genome (hg38). The Partek Flow Genomic Analysis Software was used to compare the miRNAs expression between healthy participants and patients with IgAN.