Proximity labeling of tau interactions in primary neurons and mouse brain

Microtubule-associated protein tau is a central factor in Alzheimer’s
disease and other tauopathies. However, physiological functions of tau are
unclear. Here, we used proximity labelling proteomics to chart functional
tau interactomes in primary neurons and mouse brain in vivo. Here, we use
proximity labelling with the biotin ligase BioID2 to map interactomes of
tau in neurons. Data sets relate to mass spectrometry and protein
identification of biotinylated proteins in primary neurons and in mouse
brain after delivery of BioID2-tau fusion protein or BioID2 control
protein by adeno-associated virus (AAV). Mouse brain samples are either
from P35 wild-type mice with intracranial AAV delivery at P0 or from tau
knockout mice at P60 after hippocampal delivery of AAV. Details on BioID2
fusion protein expression, biotin supplementation and sample extraction
can be obtained in the associated publication.