Identification of quiescence and early activation histones modifications pattern in satellite cells
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https://www.ncbi.nlm.nih.gov/sra/SRP116305
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资源简介：
We report that the current stem cell isolation procedures induce important transcriptional and epigenetic modifications. Focusing on adult skeletal muscle stem cells (MuSCs), here we report the epigenome (Histone 3 modifications by ChIP-sequencing) of true quiescent MuSCs and compare it to early-activated MuSCs isolated by the conventional method. Overall design: Histone 3 modifications (H3K4me3, H3K27ac, H3K27me3) ChIP-seq analysis of muscle stem cells that were either fixed in situ (T0) or isolated with the traditional dissociation protocol (T3).
创建时间：
2019-09-23
