PARAGON 2 - KM2209 - Total Organic Carbon Timecourse Incubations

This dataset contains measurements of total organic carbon concentrations (TOC) collected during the PARAGON 2 expedition (KM2209) in the North Pacific Subtropical Gyre. Measurements come from time-course incubation experiments initiated with whole seawater collected at 150 m using trace metal clean techniques and modified with various additions of organic carbon, iron, and/or nitrogen. Samples for measurements of TOC concentrations were collected at multiple time points for each experimental biological replicate by aliquoting 40 mL of whole seawater from polycarbonate incubation bottles into pre-combusted borosilicate vials. Samples were acidified with 27 µL of 12N HCl (Optima grade, Fisher), capped with Teflon-lined silicone septa lids, and stored in the dark at room temperature until analysis on shore. Total organic carbon concentrations were determined by high temperature combustion on a modified Shimadzu TOC analyzer according to Carlson et al. (2010). Timestamp is in UTC.