Data of the publication "Rapid and Differential Evolution of the Venom Composition of a Parasitoid Wasp Depending on the Host Strain"

Data files for the publication "Rapid and Differential Evolution of the Venom Composition of a Parasitoid Wasp Depending on the Host Strain." ******************************* ****** GLOBAL ANALYSIS ******************************* The compressed folder "./exemple_gel_1.zip" is an example of analysis of a gel done with CLIQS (http://totallab.com/home/cliqs/). "img_7061_converted.tif" is the analysed picture. "IMG_7061.1dxml" is the CLIQS database containing the position of the lanes, the Rf lines and the "grimaces". For details see (1). "export_without_bg" and "export_with_bg" are containing the intensity profiles exported by CLIQS and used as input for the R-functions published in (1). The file "./global_analysis_nobg_H_Q.txt" is the output of the the R-functions published in (1). This is the dataset corresponding to the global analysis of this paper. Column "nom":X[gel number]_[number of the lane in the gel, excluding the number of the lane] Columns 1 to 34: intensity of the reference bands in the corresponding lane (row in the table). Column "replicate": ID of the biological replicate of the experimental evolution. Column "generation": Generation of the experimental evolution, F1 being the hybrids of ISm and ISy. Column "hote": Host from in which the parasitoid developed (YR = resistant; SY = sensible; sen = host on which ISm and ISy are maintained: Nasr) ********************************* ****** SPECIFIC ANALYSIS ********************************* ./specific_analysis_LbGAP2.txt Column "nom": X[gel number]_[number of the lane in the gel, excluding the number of the lane] Column "replicate": ID of the biological replicate of the experimental evolution. Column "generation": Generation of the experimental evolution, F1 being the hybrids of ISm and ISy. Column "hote": Host from in which the parasitoid developed (YR = resistant; SY = sensible; sen = host on which ISm and ISy are maintained: Nasr) Column "LbGAP2_intens_western": Raw intensity of the western-blot against LbGAP2 Column "intens_mediane_global_analysis": Median intensity of the reference bands of the globale analysis used to correct the raw intensity of the western-blot against LbGAP2 (see the material and method). ./specific_analysis_LbGAP_LbSPN.txt Column "nom": X[gel number]_[number of the lane in the gel, excluding the number of the lane] Column "replicate": ID of the biological replicate of the experimental evolution. Column "generation": Generation of the experimental evolution, F1 being the hybrids of ISm and ISy. Column "hote": Host from in which the parasitoid developed (YR = resistant; SY = sensible; sen = host on which ISm and ISy are maintained: Nasr) Column "LbGAP": Presence or absence of the protein LbGAP (LbGAP is a dominant marker) Column "LbSPNm": Presence or absence of the protein LbSPNm (LbSPN is a co-dominant marker) Column "LbSPNy": Presence or absence of the protein LbSPNy (LbSPN is a co-dominant marker) (1) : Mathé Hubert, H., Gatti, J.-L., Colinet, D., Poirié, M., & Malausa, T. (2015). Statistical analysis of the individual variability of 1D protein profiles as a tool in ecology: an application to parasitoid venom. Molecular Ecology Resources, 15, 1120–1132. http://doi.org/10.1111/1755-0998.12389