Microarray analysis of NNMT overexpression and knockdown in stromal cells. Microarray analysis of NNMT overexpression and knockdown in stromal cells

Analysis of gene expression in human CAFs with control (shCtrl) or shRNA against NNMT (shNNMT) expression or normal fibroblasts expressing control (Ctrl) or NNMT overexpression (NNMT) constructs. Cells were infected with lentivirus to express the indicated shRNA construct. Hypothesis is that knockdown of NNMT will affect expression of genes via regulation of histone methylation status. Overall design: Total RNA was collected from human cancer associated fibroblasts expressing the indicated shCtrl or shNNMT constructs or from normal WI-38 fibroblasts expressing Ctrl or NNMT constructs and expression analyzed with Affymetrix microarray.