Determination of erythroid developmental status, using TER119 and CD71 markers, in cells isolated from GD16.5 liver of the three genotypes.

Percent of cells were grouped into regions R2 to R5, as shown in Fig. 6. Region 2 = proerythroblasts; region 3 = basophilic erythroblasts; region 4 = polychromatophilic erythroblasts; and region 5 = orthochromatophilic erythroblasts. TER119 = erythroid; CD71 = transferrin receptor. Classification of cells into R2 through R5 was based on previous studies [48]; [49], and the gates were applied to the total cells, without first gating on the Ter119+ population. There is a small fraction of events that do not fall into any of the gates R2 through R5, which do, however, meet the conditions for classification as TER119+.