Transcription profiling of Drosophila exposed to a levitation magnet for different lengths of time

Drosophila samples were exposed to the levitation magnet inside a 25mm diameter tubes with 3 ml of yeast-based Drosophila food in the bottom and a chamber of only 5 mm of height over the food. This small region is needed in order to guarantee that all the flies were located in the effective g area so a maximum of 35 to 40 imagos or pupa can be exposed to each condition per experiment. All experiments were carried out with a parallel 1g external control in a temperature regulated incubator outside the magnet. Three experiments of different duration were performed inside the magnet system to analyze the effect of strong magnetic fields and magnetic levitation during different stages of the Drosophila development

果蝇样本被置于直径为25毫米的管内，该管底部装有3毫升基于酵母的果蝇食物，并在食物上方设置了一个高度仅为5毫米的隔室。此小区域的存在旨在确保所有果蝇均位于有效的磁场区域，从而每个实验条件下最多可暴露35至40个成虫或蛹。所有实验均在磁场外部的温度调控孵箱中进行，并配备了一个平行1g的外部控制。在磁场系统中进行了三次不同时长的实验，以分析强磁场和磁悬浮在果蝇发育不同阶段的影响。