Effect of sex and brain region on gene expression within isolated microglia in adult C57Bl/6J mice.

We used mice that conditionally expressed a fluorescent reporter only in TMEM119 expressing cells to sort microglia for RNA-sequencing. We then performed gene expression profiling analysis using data obtained from RNA-seq of three different brain regions (prefrontal cortex, striatum, midbrain) in both male and female mice. Comparative gene expression profiling analysis of RNA-seq data for microglia isolated from prefrontal cortex, striatum, and midbrain from both male and female mice.