Immunization schedule for Experiment #2

Five monkeys in each of five vaccine groups were immunized and challenged with Pk malaria sporozoites. The four antigen vaccine given Group 1 was identical to that given to Group 1 in Experiment 1. Group 2 received only the PkCSP components of the vaccine given to Group 1. Group 3 received a ten-fold larger dose of the PkCSP DNA than Group 2 and the same dose of PkCSP COPAK. Group 4 received only the PkCSP and PkSSP2 components of the vaccine given to Group 1. Group 5, the Control group, received a mock vaccine consisting of plasmid DNA without malaria antigen inserts followed by parental COPAK without malaria inserts. (See text for more details).