Quantitative proteome analysis reveals changes of membrane proteins in Sedum plumbizincicola under cadmium stress

Sedum plumbizincicola is an herbaceous species tolerant of excessive cadmium accumulation in above-ground tissues. The implications of membrane proteins, especially integrative membrane proteins such as transporters, in Cd detoxification in plants have received attention in recent years, but a comprehensive profiling of Cd-responsive membrane proteins from Cd hyperaccumulator plants is lacking. In this study, the membrane proteins of root, stem, and leaf tissues of S. plumbizincicola seedlings treated with Cd solution for 0, 1 or 3 days were analyzed by Tandem Mass Tag (TMT) labeling-based proteome quantification. Total 3353 proteins with predicted transmembrane helices were identified and quantified in at least one tissue group