Mitochondrial genome of Zhangixalus omeimontis

Mitochondrial genomes in frogs are crucial in reconstructing phylogenetic relationships and clarifying molecular evolution in these animals. Therefore, we determined and analyzed the complete mitochondrial genome sequence of Zhangixalus omeimontis in this research. The total length of this sequence is 19,782 base pairs, containing a total of 37 genes, which include 22 tRNA genes, 13 protein-coding genes, and 2 rRNA genes, along with two D-loop regions. The mitochondrial genome exhibits a novel rearrangement pattern (tRNASer-ND6-tRNAGlu-Cytb-CR1-ND5-CR2-tRNAThr-tRNALeu-tRNAPro) of genes. The nucleotide composition of the light strand (L-strand) of the mitochondrial genome consists of 23.75% adenine (A), 34.39% thymine (T), 19.16% cytosine (C), and 22.70% guanine (G), with an overall A+T content of 63.83%, indicating a bias towards adenine and thymine. The phylogenetic tree is constructed using the Bayesian inference (BI) method. The findings indicated a close relationship between Z. omeimontis and Z. dugritei. The comprehensive mitochondrial genome of Z. omeimontis will be a valuable asset for forthcoming research endeavors focusing on the evolution, taxonomy, and genetic preservation of Zhangixalus.