Protocol for controlling visual experience during zebrafish development and modulation of motor behavior

Sensory experience instructs neurodevelopment and refines sensory processing. We describe a minimally invasive protocol to immobilize zebrafish larvae and control visual experience. Visual experience can be controlled through early development from 2-6 days post fertilization during defined intervals. This protocol avoids surgical methods necessary in other vertebrate models to control visual experience, which can be validated using readily performed and robust visuomotor assays. These methods can be adapted to broadly test the role of visual experience during development. FigureData.xlsx contains data for graphs generated for this manuscript (Figure 3F-G) post holder.stl is the file to 3D print post holder (refer to Figure 2B) Program folder contains all software for eventtimer, cplot, batchan and supporting files