Jennifer Bestman, Holly Cline (2011) CIL:37077, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This is IMAGE #6 (hour 8) of a developing neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.
The cell was imaged at a 2 hour intervals over 8 hours and shows the development and morphological plasticity of the dendritic arbor and axon over time.

The cell was transfected with in vivo electroporation of plasmid DNA encoding eGFP ~24 hours before the image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

此为来自白化非洲爪蟾(Xenopus laevis)视觉神经节神经节发育过程中的第6张图像，拍摄于第8小时。该细胞在8小时内每隔2小时进行成像，展现了树突树和轴突随时间推移的发育与形态可塑性。在图像采集前约24小时，通过活体电穿孔法将编码绿色荧光蛋白(eGFP)的质粒DNA转染至该细胞。实验中，完好的活体蝌蚪经过麻醉后置于载玻片下，并通过定制构建的双光子显微镜直接穿过其透明的皮肤进行成像。