Multi-country field data from the INDICANTS project from 2022 to 2024

These data were collected as part of the INDICANTS project, which received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 890856. The project aimed to develop point-of-care LAMP (Loop-mediated isothermal amplification) assays for field diagnosis of four vascular banana diseases:<br>
• Fusarium wilt, caused by <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (Foc)<br>
• Moko disease and banana blood disease (BDB), caused by <i>Ralstonia solanacearum</i> and <i>Ralstonia syzygii</i> subsp. <i>celebesensis</i>, respectively<br>
• Xanthomonas wilt of banana (BXW), caused by <i>Xanthomonas vasicola</i> pv. <i>musacearum</i><br>
The four LAMP protocols have been validated on field samples. MOKO disease was diagnosed by a MOKO duplex- LAMP in Jamaica and from DNA samples supplied by Ecuador. Missions in Mozambique and Indonesia have been carried out for FocTR4 LAMP, in Uganda for BXW-LAMP , Indonesia for BDB-LAMP. Samples collected in banana plantations were directly tested by LAMP in the field or in non-laboratory environment after a simplified extraction method. Reference PCR/qPCR detection were carried out by the local partners in their laboratory to compare the results. Additionally, samples from Vietnam and Comoros have been collected by the Stellenbosch University in collaboration with local partners. Stellenbosch University determined the Vegetative Compatibility. Group (VCG) of the isolated fungi, and performed both LAMP and qPCR on the samples. <br>
This table includes data for all countries on banana cultivars, their locations, the tissues sampled, and the results of LAMP and PCR/qPCR tests performed on the banana samples.