ArcAB two-component system is associated with susceptibility of Aggregatibacter actinomycetemcomitas to superoxide and hydrogen peroxide

Aggregatibacter actinomycetemcomitans is a Gram-negative facultative anaerobe and is associated with periodontal disease. A two-component system ArcAB plays a crucial role in adaptation to several conditions, but its function in A. actinomycetemcomitans remains unclear. To identify the genes regulated by the ArcA response regulator in A. actinomycetemcomitans, differentially expressed genes were analyzed using the wild-type strain NUM4039 and its isogenic mutant of arcA. The obtained data indicated that 68 genes were differentially expressed with 40 genes upregulated more than 2-fold and 28 genes downregulated more than 2-fold. Overall design: RNA-seq profiling of A. actinomycetemcomitans wild type and its inactivated mutant of arcA grown in A. actinomycetemcomitans growth medium (AAGM) broth at 37°C under ambient air supplemented with 5% CO2.