Quantitative gene expression analysis of early postnatal hippocampal development in wild-type and Pol?-deficient mice using RNA-seq

Genome stability is essential for brain development and function, as de novo mutations during neuronal development cause psychiatric disorders. However, the contribution of DNA repair to genome stability in neurons remains elusive. To examine that the base excision repair protein DNA polymerase beta (Pol?) is involved in hippocampal neuronal differentiation during early postnatal stages, gene expression profiling was performed using RNA-seq. RNA-seq analysis was carried out on hippocampal RNA extracts from P15 control Pol?fl/fl and Nex-Cre/Pol?fl/fl mice, in which forebrain postmitotic excitatory neurons lack Pol? expression.