Genome-wide binding sites of Mss11 in Candida glabrata

We found Mss11 as a transcription factor is vital for the adhesion and biofilm formation abilities of C. glabrata, and it consequently impacts its virulence. This modulation is achieved by regulating C. glabrata Epa1 and Epa6 expression. To analyze Mss11 regulation of EPA1 and EPA6 expression further, we constructed the FLAG-tagged Mss11 and conducted ChIP-seq. The results revealed that Mss11 exhibited extensive binding across all chromosomes of C. glabrata, with enriched peaks predominantly concentrated within specific genomic features. In particular, most Mss11 binding sites were located within promoter regions of the genes (89.01%), with a smaller fraction in the upstream regions (4.95%) and the remainder distributed among the exon (3.30%) and intergenic (2.75%) regions. This binding pattern aligned with the expected behavior of Mss11 as a transcription factor. Notably, our investigation revealed that Mss11 binds to approximately 2829 bp upstream of EPA1 and 2576 bp upstream of EPA6, as well as to the promoter regions of the subtelomeric silencing-related genes SIR4, RIP1, and RAP1. Chromatin immunoprecipitation DNA-sequencing(ChIP-seq) for the FLAG-tagged and the untagged Mss11 protein in Candida glabrata