Expression data from FACS-purified Lgr5-EGFP+ intestinal cells from Ubc9+/+ and Ubc9+/- mice

The Lgr5+ intestinal stem cell, Paneth and transit-amplifying cell compartment constitute the intestinal crypt which is the constant source of differentiated epithelial cells that replenish the intestinal villi ensuring organ maintenance and regeneration. The Lgr5+ crypt-based columnar (CBC) cells have been identified as the intestinal stem cells (ISCs) and, importantly, as cells-of-origin of intestinal cancer. We used microarrays to detail the global program of gene expression in normal ISCs describing a mild downregulation of the sumoylation (post-translational modification (PTM) of proteins by SUMO) imposed by the loss of one allele of the unique E2-conjugating enzyme (Ubc9) of the pathway. Lgr5-EGFPhigh, CD24middle, EpCampositive and PInegative intestinal stem cells from normal Lgr5-EGFP mice either wt for Ubc9 or heterozygous (Ubc9+/-) were sorted into RNA later solution and kept at -80ºC until further processing. 5 8-12 weeks-old mice from each group were used. 3 males and 2 females per group were used and were analysed together. RNA from more than 5000 cells was extracted using trizol and high-quality RNA was used for hybridization.