Data from: High-throughput synapse-resolving two-photon fluorescence microendoscopy for deep-brain volumetric imaging in vivo

Optical imaging has become a powerful tool for studying brains in vivo.
The opacity of adult brains makes microendoscopy, with an optical probe
such as a gradient index (GRIN) lens embedded into brain tissue to provide
optical relay, the method of choice for imaging neurons and neural
activity in deeply buried brain structures. Incorporating a Bessel focus
scanning module into two-photon fluorescence microendoscopy, we extended
the excitation focus axially and improved its lateral resolution. Scanning
the Bessel focus in 2D, we imaged volumes of neurons at high-throughput
while resolving fine structures such as synaptic terminals. We applied
this approach to the volumetric anatomical imaging of dendritic spines and
axonal boutons in the mouse hippocampus, and functional imaging of
GABAergic neurons in the mouse lateral hypothalamus in vivo.