Gene expression in alveolar macrophages between bleomycin–treated wild-type and S1pr2-/- mice.. Mus musculus

We compared mRNA expression in alveolar macrophages between bleomycin–treated wild-type and S1pr2-/- mice, using DNA microarray analysis. In S1pr2-/- macrophages, 398 genes showed decreases to less than 50% of the levels in wild-type macrophages. In contrast, 122 genes showed more than 2.0-fold increases in S1pr2-/- macrophages compared with wild-type macrophages. The downregulated genes in S1pr2-/- mice included the following potentially fibrosis–related genes: profibrotic cytokines, chemokines, and the markers characteristic of classically activated (M1) and alternatively activated (M2) macrophages. Overall design: BALF cells from wild-type and S1pr2-/- mice on day 22 after bleomycin administration were collected and analyzed. Two independent experiments were performed.