Affymetrix Gene Expression array data for Tcl1 mouse model samples

Tcl1 is known to be involved in survival, proliferation and differentiation of human lymphocytes and mouse embryonic stem cells. Loss of Tcl1 gene in the KO mouse model affects skin integrity inducing alopecia and ulcerations. The study used epidermal keratinocytes from wild type (WT), Tcl1 knock down (KO) and K14-driven TCL1 transgenic (TGKO) mouse models to investigate the role of Tcl1 gene in the skin homeostasis. Our data indicate that Tcl1 loss mainly induces a decrease in the gene expression of keratinocytes, which is largely rescued by K14-TCL1. GO analysis revealed Tcl1 to be involved in the proliferation and differentiation of mouse keratinocytes. Moreover, pathway analysis identifies growth factor and MAPK signaling to be highly implicated. Affymetrix Gene Expression arrays were performed, according to the manufacturer's directions, on RNA extracted from freshly isolated keratinocytes. Skin specimens were excised three days post depilation and epidermal sheet was separated from the dermis through incubation with 1U/mL of dispase. Gene Expression, GO and pathway analyses were performed for 3 Tcl1-/-, 3 K14-TCL1 and 2 WT samples used as references.