MeRIP-seq analysis for CSTF2 knockout in HUH7 and PLC/PRF/5 cell lines
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE268346
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CSTF2 has been shown to have a certain oncogenic effect in hepatocellular carcinoma, but its mechanism remains unclear. Previous studies have suggested that CSTF2 can shorten the 3' untranslated region (3'UTR) of target genes. Considering that the 3'UTR contains numerous m6A modification sites, we hypothesize that CSTF2 may regulate mRNA m6A modification. We performed MeRIP-seq analysis to investigate the changes in m6A modification in CSTF2 knockout HUH7 and PLC/PRF/5 cell lines. We employed CRISPR-Cas9 gene editing technology to knockout CSTF2 in HUH7 and PLC/PRF/5 cell lines. Parental HUH7 and PLC/PRF/5 cell lines were used as controls. MeRIP-seq was performed to analyze the changes in RNA methylation patterns
创建时间：
2024-05-30
