Molecular detection of viral nucleic acid by RT-PCR.
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RNA was extracted from eye swabs or PBLs and subjected to RT-PCR. The PCR product obtained by using the diagnostic primer set F1b-F2d was used as template for the nested PCR (primer set F1–F2). P = Number of animals positive for the presence of viral nucleic acid; T = Number of animals tested.
创建时间：
2015-12-02
