Correlation between sleep quantities (SWS and PS) and consequent c-Fos expression in Nesfatin-1-immunoreactive cell bodies within THA for the 4 experimental groups considered, i.e. Control, PS deprived, Bad-Sleeper and Good-Sleeper rats.

For each experimental condition, the percentage of time spent in SWS and PS scored per 5-sec epoch during the last 2 h before sacrifice is reported (mean ± SEM, n = 5 rats for Control and PS deprivation, n = 4 rats for both PS recovery samples). Then, the number (mean ± SEM) of Fos+, Nesf-1+ and Fos+/Nesf-1+ double-labeled neurons was estimated, bilaterally, for each discrete THA nucleus known to contain Nesf-1+ neurons (see Methods for detailed quantification). Here are reported quantitative data for single-labeled Fos+ and double-labeled Fos+/Nesf-1+ cell bodies when considering either the whole THA and separately, the lateral half of THA (formed by the ZI-Subl and PeF-LHA areas) which contains the majority of the THA Nesf-1+ neurons. Significance values are:*pvs. control; a pvs. privation and b pc ppost hoc test.