Z. Xu, H. Ogawa, P. Vagnarelli, J.H. Bergmann, D.F. Hudson, S. Ruchard, T. Fukagawa, W.C. Earnshaw, K. Samejima (2011) CIL:12162, Gallus gallus gallus, memory B cell. CIL. Dataset
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Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase cell localization of exogenous TrAP-INCENP (red) plus endogenous CENP-A (green) and DNA (blue) in INCENPOFF cells expressing TrAP-INCENP WT. See Fig 6A panel 9 in Xu et al. 2009 J Cell Biol 187, 637-653.
DT40鸡B淋巴细胞系细胞在有丝分裂阶段的细胞核切片。采用Deltavision系统,借助100倍/1.4NA NA计划阿波式物镜获得的宽场免疫荧光图像的Z系列,经约束迭代算法(Applied Precision)进行去卷积处理。展示的是中央Z切片。在表达TrAP-INCENP WT的INCENPOFF细胞中,呈现外源性TrAP-INCENP(红色)与内源性CENP-A(绿色)以及DNA(蓝色)的中期细胞定位。见图6A面版9,Xu等人在2009年《细胞生物学杂志》(J Cell Biol)第187卷的第637-653页。
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CIL



