RNAseq of Islet CD11c+ cells and Islet T cells from 12-20 week non-obese diabetic mice

Using intravital 2-photon islet imaging we show that T cell extravasation into the islets is an extended process, with T cells arresting in the islet vasculature in close proximity to perivascular CD11c+ cells. Antigen is not required for T cell trafficking to infiltrated islets, but T cell chemokine receptor signaling is necessary. Using RNAseq, we show that islet CD11c+ cells express over 20 different chemokines that bind chemokine receptors expressed on islet T cells. CD11c+ cells (DAPI-CD45+CD19-CD90.2-CD11c+MHCII+) and T cells (DAPI-CD45+CD90.2+) were FACS sorted from 4-8 pooled 12-20 week non-obese diabetic (NOD) mice per replicate, 4 replicates for CD11c cells and 5 replicates for T cells.