Microarray analysis of APL3 and APL4 gene expression in response to atrazine and sucrose treatments.

Microarray data for APL3 and APL4 were extracted from our previous work [12], which was carried out under similar conditions of plant growth and plant treatment as those described in Materials and Methods. The characterization of At4g39210 and At2g21590 gene products was derived from previous studies [20], [21]. Arabidopsis plantlets (Col-0 wild ecotype) were transferred to MS-agar medium in the presence of 80 mM mannitol, 80 mM sucrose, 80 mM mannitol and 10 µM atrazine, or 80 mM sucrose and 10 µM atrazine. Transcriptome analysis was carried out on pairwise comparisons [12]. Relative expressions of genes after 24 h of treatment are given as their log2(expression ratio) for sucrose versus mannitol (Suc/Mtl), mannitol plus atrazine versus mannitol (Mtl-Atrazine/Mtl) and sucrose plus atrazine versus mannitol (Suc-Atrazine/Mtl) comparisons. Statistical analysis was carried out as previously reported [12]. Genes with a Bonferroni P-value higher than 5% were considered as being not differentially expressed [63].