Localization of attachment organelle components determined by fluorescence in a living cell.

(A) Distribution of peak positions of fluorescence relative to the cell front. The cell front was defined from phase-contrast images as shown in S5 Fig. Gene identification number (ID) and protein annotation are indicated on the left. The average from 20 cells is marked by a black triangle and shown with standard deviation (SD) in each panel. The histograms are colored according to their categories shown in Fig 3 and S1 Table. (B) Fluorescence of EYFP fused to the C terminus and of ECFP fused to the N terminus of HMW2 in a cell. Fluorescence and phase-contrast images are merged. (C) Measurement of relative positions of EYFP and ECFP signals. Signal intensities along the yellow dotted line in (B) were plotted and traced as Gaussian distribution. (D) Distribution of the distances between two different proteins. Gene ID and its annotation are presented on the left with colors showing their fluorescence; EYFP (green), ECFP (cyan), and Cy3 (red). P1 adhesin was detected by immunofluorescence (IF) microscopy using a monoclonal antibody [32]. The average of 20 cells is shown with SD and marked by a black triangle in each panel.