E4orf4 inhibits DNA damage repair.

(A) Clone 13 cells were induced with Dox for 2.5 hrs to stimulate E4orf4 expression (+E4orf4), or were left uninduced (-E4orf4), and were then treated with 100 μM H2O2 for 30 minutes or were left untreated. Cell samples were then subjected to an alkaline comet assay. Representative microscopic images of the assay taken at a magnification of 200 are shown. (B). Quantitation of the comet data from A is shown. The length and intensity of DNA tails relative to heads is represented by the comet tail moment which was determined using the TriTek Comet Score software (n ≥ 100). Bars represent standard error of the mean. The statistical differences between control and E4orf4-expressing cells or between drug-treated and untreated cells are indicated with p values: *: p<0.001.