Next generation sequencing to determine changes in Drosophila Atg6 mutant larval transcriptome

Next generation sequencing was performed on third instar Drosophila larvae to determine changes in the transcriptomes of Atg6 heterozygous and Atg6 homozygous animals compared to wild type animals Overall design: RNA was isolated from Drosophila third instar larvae of the genotypes w118, w118;;Atg6-/+, and w118;;Atg6-/- using Qiagen Rneasy kit. Paired-end next generation RNA sequencing (20 milliion reads at 2 x 75bp PE) was performed in triplicate for each sample using illumina