RAC-GEF function as a critical factor in intestinal tumourigenesis

Here we investigate the transcription changes in the murine intestine 4 days following loss of APC under the control of Vil-CreErT2 driver. We compare the RNAseq data from epithelial organoids generated from control (APC loss) intestines to organoids generated from lacking VAV2, VAV3 and TIAM1. We show that loss of these GEFs supress the APC WNT driven intestinal phenotype in a RAC-dependent manner. Expression data from organoids generated from intestines from VilCreEr APCfl/fl vs VilCreER APCfl/fl Vav2-/-, Vav3-/-, Tiam1-/- mice.