TABLE 1 in A new species of planthopper in the genus Anotia (Hemiptera: Auchenorrhyncha Derbidae) from the Los Angeles cloud forest in Costa Rica
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TABLE 1. Primers used to amplify loci used for assessment of Anotia cerebro sp. n. and corresponding annealing temperatures and extension times.
GeneName/DirectionSequence (5’- 3’)AnnealingExtensionReferenceCOILCO1490/Forward C1J2195RC/ReverseGGTCAACAAATCATAAAGATATTG ACTTCTGGATGACCAAAAAATCAA40˚C1 min. 30 sec.Folmer et al. 1994 Humphries et al. 202118S18SFI/Forward 18SRI/ReverseACTGTCGATGGTAGGTTCTG GTCCGAAGACCTCACTAAA50˚C2 min.Bahder et al. 201928SV/Forward X/ReverseGTAGCCAAATGCCTCGTCA CACAATGATAGGAAGAGCC55˚C1 min. 30 sec.Cryan et al. 2000
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2025-04-05



