Figure 3a

In this experiment, we test the differences in the degradation rates between different TIR1/AFB receptors from different species. Conclusion: The maize auxin receptor ZmAFB2/3 b1 exhibited a higher basal activity compared with the Arabidopsis ortholog. A, ZmAFB2/3 b1 was sensitive to auxin and exhibited faster degradation than AtAFB2. ZmAFB2/3 a was not sensitive to auxin. Yeast coexpressing an Arabidopsis or maize auxin receptor and YFP-ZmIAA10 were treated with 1 μm auxin or mock (0), and fluorescence was measured on a flow cytometer. Notes: r1_.... means rep1 ...1_.... means plate 1 Yeast autofluorescence was cancelled out using non-fluorescent yeast. All yeast strains were tested for basal fluorescence prior to treatment from over 50 minutes of growth.