Cas12a crRNA engineering
收藏NIAID Data Ecosystem2026-03-13 收录
下载链接：
https://www.ncbi.nlm.nih.gov/sra/SRP366451
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资源简介：
Design and validation of sixteen types of structurally engineered Cas12a crRNAs targeting various immunologically relevant loci. We designed structural modifications in the loop region, ranging from engineered breaks (STAR-crRNAs) to large gaps (Gap-crRNAs), as well as nucleotide substitutions, enable gene-cutting in the presence of various Cas12a nucleases. Moreover, we observe similar insertion rates of short HDR templates using the engineered crRNAs compared to the wild-type crRNAs, further demonstrating that the introduced modifications in the loop region lead to comparable genome editing efficiencies.
创建时间：
2022-05-31
