The degree of H3.1 or H3.3 mobilization correlates with the progression of infection.

(A) Average normalized levels of free GFP-H3.1 relative to mock-infected cells at 4 or 7 hpi, respectively. Vero cells were transfected with plasmids expressing GFP-H3.1 (H3.1) or -H3.3 (H3.3). Transfected cells were mock-infected or infected with 30 PFU/cell of strain n212. Mobilization of GFP-H3.1 or -H3.3 was examined from 4 to 5 (4) or 7 to 8 (7) hpi by FRAP; error bars, SEM; dashed line, normalized average level of free GFP-H3.1 in mock-infected cells. (B) Frequency distribution plots of the percentage of free GFP-H3.1 per individual cell at 4 or 7 hpi; dotted line, one SD above the average level of free GFP-H3.1 in mock-infected cells. (C) Average initial rate of normalized fluorescence recovery relative to mock-infected cells at 4 hpi; error bars, SEM. (D) Frequency distribution plots of the initial rate of normalized fluorescence recovery of GFP-H3.1 per individual cell; dashed lines, one SD below the average initial rate of normalized fluorescence recovery in mock-infected cells (not visible because they are at 0 and overlap the y-axis). (E) Average normalized levels of free GFP-H3.3 relative to mock-infected cells at 4 or 7 hpi; error bars, SEM; dashed line, normalized average level of free GFP-H3.3 in mock-infected cells. (F) Frequency distribution plots of the percentage of free GFP-H3.3 per individual cell at 4 or 7 hpi; dotted line, one SD above the average level of free GFP-H3.1 in mock-infected cells. (G) Average initial rate of normalized fluorescence recovery relative to mock-infected cells at 4 hpi; error bars, SEM. (H) Frequency distribution plots of the initial rate of normalized fluorescence recovery of GFP-H3.3 per individual cell; dotted line, one SD above the average initial rate of normalized fluorescence recovery in mock-infected cells. **, P<0.01; *, P<0.05; n.s., not significant.