THE USE OF NEXT GENERATION SEQUENCING TO SOLVE PHYLOGENETIC INCONGRUENCES IN ORCHIDACEAE. Polystachya phylogeny
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https://www.ncbi.nlm.nih.gov/bioproject/PRJEB23182
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We used the almost complete chloroplast genome of 48 individuals, which are form a clade within the genus. The method consisted in construct genomic libraries, hybridize it with RNA probes designed from available sequences of a related species, and subsequent sequencing of the product. Altogether, we analyzed 131,214 bp, between coding and non-coding regions of the chloroplast genome. The phylogeny was able to resolve the relationship between the selected clade with high support values.
创建时间:
2017-12-26



