THE USE OF NEXT GENERATION SEQUENCING TO SOLVE PHYLOGENETIC INCONGRUENCES IN ORCHIDACEAE. Polystachya phylogeny

We used the almost complete chloroplast genome of 48 individuals, which are form a clade within the genus. The method consisted in construct genomic libraries, hybridize it with RNA probes designed from available sequences of a related species, and subsequent sequencing of the product. Altogether, we analyzed 131,214 bp, between coding and non-coding regions of the chloroplast genome. The phylogeny was able to resolve the relationship between the selected clade with high support values.