Seasonal changes of gene expression of eyes in Medaka fish (Oryzias latipes)

To examine the seasonal adaptation, we compared the gene expression of eyes between SL (short-day and low-temperature conditions: 10 h light/14 h dark and 8 °C) and LD (long-day and warm-temperature conditions: 14 h light/10 h dark and 26 °C) conditions in Medaka fish (Oryzias latipes). The gene expression of eyes was measured at 16 hours after light onset (zeitgeber time: ZT16) on days 0, 1, 2, 3, 5, 7, and 14 after transfer from SL to LD conditions. For each time point, cRNAs prepared from pooled eyes (n=3) were hybridized to duplicate sets of microarrays.