RNA-seq: STAT1 and STAT3 transcription factors sense and interpret changes in inflammation through engagement of specific enhancers

To investigate potential changes in the provision of competent host defence versus inflammation-induced tissue injury, we used RNA-seq to profile gene signatures in 'resolving' and 'pro-fibrotic' peritoneal lining tissues. We performed RNA-seq in peritoneal membranes from WT and IL6KO mice challenge with SES - Lyophilized cell-free supernatant prepared from Staphylococcus epidermidis ('resolving') and mice challenged with SES and CD4+ Th1 cells at the same time ('pro-fibrotic'). The peritoneal membrane was harvested at 3h and 6h after the injection.