Solution structure of nucleases from three staphylococcal species and the high temperature and salt tolerance by SAXS

Nuclease has important application in modern therapy biotechnologies which, however, requires salt tolerant and active at a broad range of temperature. To achieve such a “universal” nuclease, the relations between these properties and the primary sequence is needed. The aim of the project is using SAXS to determine and compare the folding/unfolding as well as monomeric/oligomeric state of three staphylococcal nucleases in dependence to increasing temperature (up to 50 oC) and NaCl concentration (up to 1M).