mRNAseq in SBMA and control iPSC-derived motor neurons

RNA sequence was performed using mRNAs of motor neurons derived from iPSCs of four patients of spinal bulbar muscular atrophy (SBMA) and four age- and sex- matched controls. The analysis was performed using purified motor neurons by flowcytometry and cell sorting based on the expression of HB9e438::Venus reporter gene (P4) or unpurified motor neurons (NT). mRNA profiles obtained from motor neurons derived from four SBMA-iPSCs and four control-iPSCs, each of the group has NT and P4 fraction, were generated by deep sequencing using Illumina NextSeq500.