RNA-seq in control and Haster-deficient mouse livers

The HASTER promoter region is a cis-regulatory element that stabilizes the transcription of HNF1A, preventing silencing or overexpression. We have generated a mouse model where the promoter of Haster has been specifically deleted in liver (Haster loxP/loxP; AlbCre). In liver the prevailing consequence is upregulation of HNF1A. We performed RNA-seq from control and KO livers to assess the transcriptional impact of the HNF1A upregulation in the Haster KO liver.HASTER启动子区域（HASTER promoter region）是一类顺式调控元件（cis-regulatory element），可稳定HNF1A的转录，避免其发生基因沉默或异常过表达。本研究构建了肝脏特异性敲除Haster启动子的小鼠模型（Haster loxP/loxP; AlbCre）。在该模型的肝脏组织中，最主要的效应为HNF1A表达上调。我们对对照组与敲除组肝脏样本进行了RNA测序（RNA-seq），以此评估Haster敲除肝脏中HNF1A上调所带来的转录组影响。