The ER does not undergo major changes following microtubule depolymerization.

A. Control experiment demonstrating the depolymerization of MTs (green) upon treatment with benomyl. Unpolymerized TubA-GFP accumulates in the cytosol, leaving the nuclei empty (nuclei are labeled with HhoA-mCherry). The picture was taken after after 17 min of incubation in the presence of the drug. B. Effect of benomyl treatment on the Sec63-GFP-labeled ER. The representative example shown on the right corresponds to a cell incubated for 30 min in the presence of benomyl. The positions of the nuclei are revealed by the HhoA-mCherry fluorescence.