Mapping-by-sequencing of the sin mutation

To identify the mutation that underlies the sin locus, we performed mapping-by-sequencing on an F2 population derived from the cross between a sin mutant and a wild tomato Solanum pimpinellifolium plant (accession LA1589). Genome-wide analysis of the allele frequencies in two pools containing 15 mutant and 15 randomly selected wild-type plants allowed us to identify the genomic region harboring the causal mutation of the sin phenotype.