Single-cell transcriptome dynamics in stabilized pituitary gonadotrope cells [10x]

Studying dynamic transcripts in single cells (SC) requires large numbers of timed samples. We report an easy to use protocol to stabilize RNA in intact SCs without perturbing transcriptional patterns, and demonstrate its applicability for SC transcriptome assays with cells and tissue. We identify a gene-specific hierarchical pattern of all-or-none transcript induction elicited by different concentrations of pulsatile hormone stimuli in pituitary gonadotropes. GEM Drop-seq was performed on two samples of LβT2 cells preserved with RNA-Best: one sample was treated with vehicle (Control), the other with 2nM GnRH for 40 min.