Microarray gene expression data in control and DGCR8-depleting human mesenchymal stem cell

To determine the miRNAs potentially responsible for the premature-senescence mediated gene expression regulation in human adipose-derived mesenchymal stem cells, we performed illumine bead microarray analyses on parental hMSCs, and globally depleted miRNAs by inhibiting DGCR8, an essential component of miRNA biogenesis. DGCR8 knockdown in hMSCs resulted in severe proliferation defects with senescence-associated changes including markedly increased reactive oxygen species (ROS) levels. In gene expression profiling, thousand two hundred and forty seven transcripts were upregulated and 1,136 genes were downregulated in Dgcr8 knockdown cells compared to control cells (p < 0.05, Fisher’s exact test). Adipose-derived human mesenchymal stem cells were incubated at 37°C in 5% CO2 and grown to 50-60 % confluence, transfected with siRNAs against DGCR8 (siDGCR8) and GFP control (siGFP;ST Pharm) at a concentration of 32 nM using Lipofectamine 2000 (Invitrogen). Replicative senescence mediated gene expression regulation in early (PDL; 3~5) and late passage (PDL; 15~40) human dipose-derived mesenchymal stem cells